This course will provide hands-on training in genome editing and cell engineering in mammalian cells using the highly efficient CRISPR/Cas9 system. Participants will learn design of CRISPR targets using bioinformatics tools, generation of gene knockouts/knock-ins, and target validation using the most current course is aimed at researchers who are familiar with basic molecular and cell biology techniques and want to learn how to create an engineered mammalian cell lines using the most recent and advanced CRISPR/Cas9 system. No previous experience in genome editing is required.