A rapidly increasing number of researchers aim to purify and identify extracellular vesicles and many methodologies to isolate and analyse these vesicles are available and have been implemented. This course will cover the basics, possibilities and limitations of different purification and characterization techniques and will address strategies to understand their role in biomedical applications. This course is intended for PhD students and Postdoctoral researchers who are interested in learning how to work with extracellular vesicles and know more about their biomedical applications. In this course we intend to make PhD students and post-doctoral researchers familiar with good-practice-guidelines and methods for purification and identification of extracellular vesicles with practical sessions included.