Recent advances in optical microscopy have transformed neuroscience, providing an unprecedented view of the structure and function of neural systems. This conference will explore current developments in microscopy and related biophysical and computational methods for measuring and controlling the nervous system, from molecules to entire neural circuits. Co-chairs:Valentina Emiliani (Neurophotonics Laboratory, CNRS-Paris Descartes University, France)&Karle Svoboda (Janelia Research Campus, Howard Hughes Medical Institute, USA)The Brain Conferences establish a series of high-level meetings on neuroscience in Europe. Organised by FENS in collaboration with The Brain Prize, these bi-annual conferences bring together outstanding researchers in key areas of contemporary neuroscience to discuss current concepts and define challenges for future research.